Anti-MSRA (PMSR) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UJ68

Core Product Specifications and Parameters

Biological Background: MSRA Function and Localization

• MSRA (PMSR) is a mitochondrial and cytosolic enzyme that acts as a peptide methionine sulfoxide reductase, catalyzing the reversible reduction of methionine sulfoxide residues in proteins back to methionine. This repair function is critical for reversing oxidative damage in proteins.
  Related references: PMID:10452521 PMID:12039877
• The protein is also known as PMSR, peptide-methionine (S)-S-oxide reductase, or protein-methionine-S-oxide reductase, and the gene is designated MSRA. Alternative splicing and alternative promoter usage generate multiple isoforms.
• MSRA localizes to multiple subcellular compartments, primarily the mitochondrial matrix, but also the cytoplasm, nucleus, and membrane. This distribution reflects its broad role in protecting cellular proteins from oxidative stress.
• The enzyme is ubiquitously expressed with highest levels in adult kidney, cerebellum, liver, heart ventricles, bone marrow, and hippocampus, highlighting its importance in tissues with high metabolic activity.
• Key structural features include a redox-active center and disulfide bond formation essential for catalytic activity. The protein undergoes post-translational modifications such as acetylation and myristoylation, which may regulate localization and function.
• UniProt lists a molecular weight of 26,132 Da, consistent with the observed ~26 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The polyclonal antibody is generated against a recombinant immunogen spanning amino acids 24–235; it is expected to detect multiple isoforms. Consider validating in lysates from tissues with high MSRA expression (e.g., kidney, cerebellum) for WB, and use appropriate loading controls.
• For IF/ICC, the antibody’s recognition of MSRA in mitochondria and cytoplasm suggests co‑staining with organelle markers (e.g., MitoTracker, DAPI) may help confirm localization. Fixation and permeabilization conditions should be optimized.
• In ELISA assays, titrate the antibody to determine the optimal working concentration, and include appropriate negative controls. Cross‑reactivity with mouse and rat enables use in rodent model studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, technical services, and supply solutions. This MSRA antibody is part of our oxidative stress and aging research portfolio, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Partner with CamelBio to streamline your IVD development from concept to clinic.