Anti-MSR1 Rabbit Polyclonal Antibody for WB and ELISA - P21757

Core Product Specifications and Parameters

Biological Background: MSR1 Function and Localization

• Receptor overview: MSR1, also known as CD204 or SCARA1, is a macrophage scavenger receptor that mediates the binding, endocytosis and phagocytosis of diverse ligands. It belongs to the class A scavenger receptor family and serves as a pattern recognition receptor.
• Modified LDL uptake and foam cell formation: The receptor recognizes and internalizes modified low-density lipoproteins, including acetylated LDL (AcLDL) and oxidized LDL (OxLDL), promoting cholesterol accumulation and contributing to foam cell formation during atherogenesis.
  Related references: PMID:2251254 PMID:34252459
• Innate immune clearance: MSR1 binds pathogen-associated molecular patterns and damage-associated molecules, such as bacterial cell wall components and apoptotic cell debris, facilitating macrophage-mediated clearance in innate immunity.
  Related references: PMID:10722588
• CpG DNA‑TLR9 signaling: Upon CpG DNA stimulation, MSR1 triggers LYN phosphorylation, which then phosphorylates SYK that is recruited to endosomal TLR9, thereby enhancing downstream innate immune signaling.
  Related references: PMID:29057947 PMID:40980882
• Subcellular localization: MSR1 is a transmembrane protein anchored at the cell membrane.
• Tissue expression: The receptor is expressed in monocyte-derived macrophages, with specific isoforms (I and II) additionally detected in liver, placenta and brain.
• Isoform diversity: Multiple isoforms are generated by alternative splicing; one isoform does not internalize acetylated LDL, highlighting functional differences.
• Post‑translational modifications: The protein is glycosylated and phosphorylated, and its structure includes coiled-coil, collagen-like, signal-anchor, and transmembrane helical regions, along with disulfide bonds.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fusion protein spanning amino acids 192-451 of human MSR1 (NP_619729.1). This region lies within the extracellular portion, likely ensuring recognition of native surface receptor.
• Western blotting: A band at approximately 50 kDa is expected. Due to glycosylation and isoform variability, bands of slightly higher molecular weight may be observed. Optimize blocking and antibody dilution in your specific sample type.
• ELISA: The polyclonal format provides robust signal for sandwich or direct ELISA setups. Consider pairing with a monoclonal capture antibody for improved specificity.
• Cross‑reactivity: The antibody recognizes human, mouse and rat MSR1, making it suitable for cross‑species studies. Verify performance in your model system.
• Sample preparation: For macrophage lysates, use standard RIPA buffer containing protease and phosphatase inhibitors to preserve epitope stability and phosphorylation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing services. Our MSR1 antibody supports research into macrophage biology, atherosclerosis, and innate immunity, and we can tailor sourcing solutions to meet your diagnostic or research manufacturing needs.