Anti-mSin3A Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q96ST3

Core Product Specifications and Parameters

Biological Background: mSin3A Function and Localization

• mSin3A is a 145 kDa paired amphipathic helix protein that functions as a transcriptional corepressor, serving as a scaffold for histone deacetylase complexes to mediate gene silencing.
• It acts as a corepressor for the RE1-silencing transcription factor (REST) and interacts with MXI1 to antagonize MYC-mediated oncogenic activities, thereby repressing MYC-responsive genes.
• mSin3A cooperates with O-GlcNAc transferase (OGT) to repress transcription in parallel with histone deacetylation, linking epigenetic regulation to nutrient sensing.
• The protein is required for the transcriptional repression of circadian clock target genes (e.g., PER1) by the PER complex, contributing to the control of circadian rhythms.
• mSin3A localizes to the nucleus and nucleolus, consistent with its role in chromatin remodeling and transcription regulation.
• It is highly expressed in the developing brain, particularly in the ventricular zone of various cortical regions, and is essential for cortical neuron differentiation and callosal axon elongation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may modulate its repressor activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1174–1273 of human mSin3A, targeting the C‑terminal region; this design supports specificity for the intended epitope.
• For IHC-P, standard antigen retrieval methods are recommended, and nuclear staining should be expected given the protein’s localization.
• In IF/ICC, permeabilization is required; anticipate nuclear and nucleolar signal patterns consistent with mSin3A distribution.
• For ELISA, the immunizing peptide can be used as a positive control to validate assay performance.
• This antibody cross-reacts with mouse and rat mSin3A, but users should verify reactivity in their specific model systems.
• Note that this product is not validated for Western blotting; if WB detection is needed, an independent validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high‑quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti‑mSin3A monoclonal antibody supports studies in transcriptional regulation, epigenetics, and circadian biology. For custom requirements or rare target sourcing, our team offers comprehensive technical consultation and supply chain solutions.