Anti-MSH6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P52701

Core Product Specifications and Parameters

Biological Background: MSH6 Function and Localization

• MSH6, also known as GTBP or p160, is the 160 kDa subunit of the MutS alpha mismatch repair complex.
• Partners with MSH2 to form MutS alpha, which initiates post-replicative DNA mismatch repair (MMR) by binding single base mismatches and small insertion-deletion loops.
• Upon DNA mismatch recognition, the MutS alpha complex bends DNA and undergoes an ATP-dependent conformational switch to become a sliding clamp, enabling downstream repair processes.
• Recruited to chromatin in G1 and early S phase via its PWWP domain binding to trimethylated histone H3 Lys36 (H3K36me3), ensuring early MMR at replication forks.
• Also implicated in DNA homologous recombination repair.
• Localizes to the nucleus and chromosomes.
• Post-translationally modified by acetylation and phosphorylation; mutations in MSH6 are associated with Lynch syndrome (hereditary nonpolyposis colorectal cancer, HNPCC).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1‑290); polyclonal reactivity should be validated in the relevant biological sample system.
• For Western blot, the expected molecular weight is ~153 kDa; consider using appropriate protein ladders and loading controls.
• IHC‑P and IF/ICC applications may require antigen retrieval and appropriate fixation/permeabilization steps.
• Cross‑reactivity with mouse and rat MSH6 makes this antibody suitable for translational studies, but researchers should perform their own species‑specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and clinical laboratories with streamlined access to a comprehensive portfolio of IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. Whether you need this anti‑MSH6 antibody for DNA mismatch repair assay development or require custom antibody solutions, our one‑stop supply chain supports every stage from concept to clinic.