Anti-MSH6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P52701

Core Product Specifications and Parameters

Biological Background: MSH6 Function and Localization

• MSH6 encodes the DNA mismatch repair protein Msh6, also known as GTBP, GTMBP, or p160, a crucial component of the post-replicative mismatch repair (MMR) system.
• Msh6 heterodimerizes with MSH2 to form MutS alpha, the complex that recognizes single base mismatches and small insertion-deletion loops (IDLs) in DNA.
• Upon mismatch binding, MutS alpha undergoes ATP-dependent conformational changes, transitioning into a sliding clamp that diffuses along the DNA backbone to signal repair.
• Recruited to chromatin in G1 and early S phase via its PWWP domain, which binds trimethylated 'Lys-36' of histone H3 (H3K36me3), enabling rapid mismatch detection during replication.
• Localized predominantly in the nucleus and on chromosomes, consistent with its role in maintaining genomic integrity.
• Mutations in MSH6 are associated with Lynch syndrome (hereditary nonpolyposis colorectal cancer, HNPCC5) and a predisposition to various cancers.
• Post-translational modifications, including acetylation and phosphorylation, and alternative splicing generate multiple isoforms that may fine-tune MMR activity.
• In addition to mismatch repair, MutS alpha may participate in DNA homologous recombination repair, underscoring its multifaceted role in genome maintenance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human MSH6; this sequence is conserved among primates and rodents, consistent with the observed cross-reactivity.
• For Western blotting, a band of approximately 153 kDa is expected; consider running appropriate molecular weight markers and verifying specificity with knockout or siRNA controls.
• When using in immunohistochemistry (IHC-P), test antigen retrieval methods (e.g., heat-induced epitope retrieval) and optimize antibody dilutions on positive and negative control tissues.
• For immunofluorescence (IF/ICC), assess fixation and permeabilization conditions; MSH6 nuclear localization should be prominent, with potential chromatin association.
• In ELISA applications, the antibody may be used for capture or detection; validate pairing with other MSH6 antibodies if developing a sandwich assay.
• As a polyclonal antibody, lot-to-lot consistency may vary; inclusion of a positive control (e.g., HeLa cell lysate) and a negative control (e.g., MSH6 knockout cells) is recommended to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-MSH6 antibody supports research into DNA mismatch repair and cancer diagnostics, connecting you to a reliable supply network from concept to clinic. Our streamlined sourcing bridges the gap between innovative research and diagnostic development.