Anti-MSH4 Rabbit Polyclonal Antibody for WB and ELISA - O15457

Core Product Specifications and Parameters

Biological Background: MSH4 Function and Localization

• MSH4 (MutS protein homolog 4) is a member of the MutS family of DNA mismatch repair proteins, but it specifically functions in meiotic recombination rather than DNA mismatch repair.
• It plays a critical role in reciprocal recombination and proper segregation of homologous chromosomes during meiosis I.
• MSH4 is highly expressed in testis and also expressed in ovary, reflecting its essential function in gametogenesis.

Related references: PMID:33437391 PMID:9299235

• The protein localizes to chromosomes, consistent with its direct involvement in meiotic recombination and chromosome dynamics.
• MSH4 contains conserved ATP-binding and DNA-binding domains that are crucial for its activity in homologous recombination.
• Mutations in MSH4 are associated with human reproductive disorders, including premature ovarian failure (POF20) and spermatogenic failure (SPGF2).
• The molecular weight of MSH4 is approximately 105 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 697–936) of human MSH4, which is predicted to be specific for MSH4 among MutS family members. Cross-reactivity with mouse and rat has been confirmed.
• For Western blotting, the expected molecular weight is around 105 kDa. Use appropriate controls and account for possible post-translational modifications.
• For ELISA, consider checkerboard titrations and validate with recombinant MSH4 protein or cell lysates from testis or ovary tissues.
• Given its role in meiosis, expression levels may be low in somatic cells; thus, enriching meiotic cells or using overexpression systems may be beneficial.
• As a polyclonal antibody, lot-to-lot variation can occur; validate each new lot in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, from concept to clinic. Our catalog includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-MSH4 antibody supports research into meiotic recombination and related reproductive disorders, and our team can assist with sourcing other key targets in reproductive biology and beyond.