Anti-MS4A8B Polyclonal Antibody for WB, ELISA - Q9BY19

Core Product Specifications and Parameters

Biological Background: MS4A8B Function and Localization

• MS4A8B (Membrane-spanning 4-domains subfamily A member 8B), also termed Four-span transmembrane protein 4 (4SPAN4) or CD20L5, belongs to the MS4A gene family of four-transmembrane proteins.
• The protein is predicted to function as a component of a multimeric receptor complex, potentially mediating signal transduction processes (UniProt: Q9BY19).
• MS4A8B contains four transmembrane domains and is classified as an integral membrane protein.
• Expression is predominantly observed in hematopoietic tissues and cell lines, indicating a role in immune cell regulation.
• UniProt keywords include Membrane, Receptor, and Transmembrane helix, underscoring its structural and functional features.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using lysates from hematopoietic cell lines (e.g., Jurkat, Raji, or THP-1) where MS4A8B is endogenously expressed; the predicted molecular weight is ~26 kDa.
• The immunogen spans amino acids 100-200; thus, the antibody may detect both native and denatured forms; validate detection under reducing and non-reducing conditions in your experimental system.
• In ELISA applications, coat the plate with the immunogen peptide or a recombinant MS4A8B protein fragment, and optimize primary and secondary antibody dilutions to minimize background.
• As with any polyclonal antibody, include positive and negative controls to confirm specificity and lot consistency, and titrate the antibody for optimal performance in your assays.

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CamelBio is your one-stop IVD raw-material partner, providing validated antibodies like this anti-MS4A8B polyclonal antibody for hematopoietic and signal transduction research. Explore our comprehensive portfolio, including optimized antibody pairs, bulk ancillary reagents, and rare target sourcing. All backed by expert technical support to streamline your diagnostic development from concept to clinic.