Anti-MS4A14 Rabbit Monoclonal Antibody for WB, ELISA - Q96JA4

Core Product Specifications and Parameters

Biological Background: MS4A14 Function and Localization

• MS4A14, also known as MS4A16 or NYD-SP21, is a member of the membrane-spanning 4-domains subfamily A, characterized by four transmembrane helical regions.
• The protein localizes to the cell membrane, consistent with its predicted structure as an integral membrane protein.
• Functional studies suggest MS4A14 may participate in signal transduction as part of a multimeric receptor complex.
• Alternative splicing generates multiple transcript variants, potentially encoding isoforms with distinct regulatory roles.
• Initially identified as a testis development protein (NYD-SP21), it is implicated in male germ cell development and reproductive biology.
• Database annotations highlight its classification as a membrane receptor with transmembrane helix topology, belonging to the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (residues 150–280) of human MS4A14. For Western blot, use a whole-cell lysate or membrane-enriched fraction from human or rat tissues/cell lines. A band near 77 kDa is expected; consider including a positive control expressing the target.
• For ELISA, the antibody can be employed to capture or detect recombinant MS4A14 protein; validate coating and detection conditions with purified antigen.
• Cross-reactivity with rat protein is reported; verify suitability for your specific rat sample and application.
• Since MS4A14 is a multi-pass membrane protein, mild denaturing conditions (e.g., reducing but not boiling) may help preserve epitope accessibility during sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to IVD raw materials. For studies involving membrane receptors and signal transduction, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies like this MS4A14 clone, and bulk ancillary reagents. Whether you are developing immunoassays or exploring rare target sourcing, our team can assist with tailored procurement solutions.