Anti-MRRF Monoclonal Antibody for WB, ELISA - Q96E11

Core Product Specifications and Parameters

Biological Background: MRRF Function and Localization

• MRRF (Mitochondrial Ribosome-Recycling Factor), also known as RRF or MRFF, is a mitochondrial protein responsible for the disassembly of ribosomes from mRNA at the termination of mitochondrial translation. Related references: PMID:19716793, PMID:33878294
• It acts in collaboration with GFM2 (mitochondrial elongation factor G2) to promote ribosome recycling by dissolution of intersubunit contacts. Related references: PMID:33878294
• Encoded by the MRRF gene (GeneID: 92399) in humans, the protein localizes to the mitochondrion and contains a transit peptide directing it to the organelle.
• Alternative splicing of MRRF may yield different isoforms, as indicated by the UniProt keyword.
• The protein is involved in the essential process of mitochondrial protein biosynthesis, ensuring efficient turnover of ribosomes.
• Its 3D structure has been determined, providing insights into its molecular mechanism.
• MRRF is ubiquitously expressed and conserved across species, with cross-reactivity observed in human, mouse, and rat samples.

Experimental Guidance and Technical Tips

• For Western blot, consider using mitochondrial-enriched fractions or whole-cell lysates from human, mouse, or rat cells. Since the immunogen is a C-terminal peptide (aa 163-262), the antibody may recognize both full-length and potential alternatively spliced isoforms.
• In ELISA, coat with recombinant MRRF protein or cell lysates; validate assay conditions for your specific matrix.
• Always include appropriate controls and validate the antibody's performance in your experimental system before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to critical IVD raw materials. This anti-MRRF monoclonal antibody exemplifies our commitment to supplying high-quality tools for mitochondrial and translational research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and specialized sourcing for rare targets—supporting your project from concept to clinic.