Anti-MRPS9 Polyclonal Antibody for WB, IHC-P, ELISA - P82933

Core Product Specifications and Parameters

Biological Background: MRPS9 Function and Localization

• MRPS9 encodes the mitochondrial ribosomal protein S9 (also known as uS9m, MRP-S9, S9mt), a component of the small 28S subunit of the mitochondrial ribosome.
• As a mitochondrial ribosomal protein, MRPS9 plays an essential role in mitochondrial protein translation, contributing to the synthesis of 13 polypeptides encoded by mitochondrial DNA.
• Belongs to the universal ribosomal protein uS9 family, conserved across prokaryotes and eukaryotic organelles.
• Localizes exclusively to the mitochondrion, consistent with its function in the organelle's translation machinery.
• Post-translational modifications include acetylation, as evidenced by proteomics identification.
• UniProt record highlights additional keywords: 3D-structure, mitochondrion, reference proteome, ribonucleoprotein, and transit peptide, indicating its import into mitochondria and structural characterization.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fusion protein spanning amino acids 237–396 of human MRPS9. The predicted molecular weight is ~46 kDa, consistent with UniProt entry (45.8 kDa); a band near 46 kDa is expected in western blotting.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and validate in the appropriate tissue sample systems, as mitochondrial proteins may require optimized permeabilization.
• Polyclonal antibodies may exhibit lot-to-lot variation; titrate the antibody for each application.
• Recommended for ELISA detection and quantification of MRPS9 in mitochondrial extracts or recombinant protein assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with validated IVD raw materials for mitochondrial and translational biology research. For projects targeting mitochondrial ribosomes or energy metabolism, we offer comprehensive sourcing of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. Whether you need bulk raw materials or rare-target antibody supplies, CamelBio’s one-stop platform bridges your research from concept to clinic.