Anti-MRPS7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2R9

Core Product Specifications and Parameters

Biological Background: MRPS7 Function and Localization

• MRPS7 encodes the mitochondrial small ribosomal subunit protein uS7m, also known as 28S ribosomal protein S7, MRP-S7, S7mt, and bMRP27a.
• As a component of the mitochondrial ribosome, MRPS7 is essential for the translation of 13 mitochondrial DNA-encoded proteins involved in oxidative phosphorylation.
• MRPS7 is located in the mitochondrion, where it participates in the assembly and function of the small (28S) ribosomal subunit.
• Post-translational modifications include acetylation, which may regulate ribosomal activity or stability.
• Disease-associated variants in MRPS7 have been linked to primary mitochondrial disease (COXPD34), highlighting its critical role in mitochondrial function.
• The protein has been identified through proteomics studies and is part of the human reference proteome, with a molecular weight of approximately 28 kDa.
• Three-dimensional structural data is available for MRPS7, providing insights into its ribosomal integration and interaction network.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 110–190) of human MRPS7; confirm the epitope accessibility in your specific assay conditions.
• For Western blotting, use mitochondrial-enriched lysates or whole cell lysates from human, mouse, or rat tissues; a specific band near 28 kDa is expected.
• For immunofluorescence/ICC, methanol or formaldehyde fixation followed by permeabilization is recommended to detect mitochondrial localization; co-stain with a mitochondrial marker.
• Validate the antibody's cross-reactivity in your target species and model system, as the immunogen shows high sequence identity between human, mouse, and rat.
• ELISA applications may require optimization of coating and detection conditions; consider using recombinant MRPS7 fragments as standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between raw-material discovery and diagnostic development, offering validated MRPS7 antibodies, matched antibody pairs, and ancillary reagents for mitochondrial biomarker research. As a one-stop sourcing partner, we streamline procurement of bulk monoclonal and polyclonal antibodies, recombinant proteins, and rare-target raw materials to accelerate your IVD and translational projects. Contact us to discuss custom sourcing solutions for your specific assay development needs.