Anti-MRPS7 Polyclonal Antibody for WB, ELISA - Q9Y2R9

Core Product Specifications and Parameters

Biological Background: MRPS7 Function and Localization

• MRPS7 (also known as MRP-S7, bMRP27a, S7mt) is a mitochondrial ribosomal protein that constitutes an integral component of the small 28S subunit of the mitoribosome.
• The MRPS7 gene encodes a protein with a molecular weight of approximately 28 kDa that localizes to the mitochondrial matrix, where it participates in the translation of mitochondrially encoded mRNAs.
• As a member of the universal ribosomal protein uS7 family, MRPS7 shares structural homology with bacterial S7 and is essential for the assembly and function of the mitochondrial ribosome.
• Post-translational modifications identified by proteomics include acetylation, which may influence protein stability or ribosome activity.
• Mutations in MRPS7 have been linked to primary mitochondrial disease, highlighting its critical role in mitochondrial homeostasis and energy metabolism.
• The protein contains a mitochondrial transit peptide at its N-terminus that directs its import into the mitochondria after cytosolic synthesis.
• Alternate names such as MRP-S7, bMRP27a, and S7mt reflect its dual nomenclature in mitochondrial and ribosomal research contexts.

Experimental Guidance and Technical Tips

• The immunogen was designed to target a specific peptide sequence (aa 40–140) of human MRPS7; consider verifying reactivity with endogenous protein in relevant mitochondrial fractions.
• For Western blot analyses, a band at approximately 28 kDa is expected; validate antibody specificity using mitochondrial lysates or cells with modulated MRPS7 expression.
• The antibody is reported as KD validated (knockdown validated), suggesting prior testing in siRNA-mediated knockdown experiments; it is advisable to confirm performance in your own cellular model.
• In ELISA applications, optimization of coating antigen (e.g., recombinant MRPS7 or peptide) and antibody dilution is recommended to achieve optimal signal-to-noise ratios.
• Because MRPS7 is a nuclear-encoded mitochondrial protein, consider subcellular fractionation to enrich the target and reduce background in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing convenient access to high-quality antibodies like this anti-MRPS7 polyclonal antibody for mitochondrial and disease research. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, enabling you to accelerate assay development. Whatever your sourcing needs—from rare target reagents to custom production—CamelBio supports your journey from concept to clinic with tailored supply solutions.