Anti-MRPS6 Rabbit Polyclonal Antibody for WB and ELISA - P82932

Core Product Specifications and Parameters

Biological Background: MRPS6 Function and Localization

• MRPS6 (Mitochondrial Ribosomal Protein S6) is a component of the mitochondrial small ribosomal subunit (28S), classified as protein bS6m in the standard nomenclature for mitochondrial ribosomal proteins.
• As an integral part of the mitoribosome, MRPS6 contributes to the structural framework and decoding site, facilitating mitochondrial protein synthesis essential for oxidative phosphorylation.
• The MRPS6 gene is located on human chromosome 21q22.11 and encodes a single protein product without known isoforms.
• MRPS6 is exclusively localized within the mitochondria, imported post-translationally into the organelle to assemble with ribosomal RNA and other proteins.
• According to UniProt, the protein has been structurally characterized (keyword: 3D-structure), and has been identified in large-scale proteomics studies.
• The small subunit of the mitochondrial ribosome is evolutionarily related to bacterial ribosomes, and MRPS6 is the mammalian homolog of bacterial ribosomal protein S6.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers the N-terminal 125 residues of MRPS6, which may contribute to its cross-reactivity across human, mouse, and rat species.
• For Western blot (WB) analysis, consider using mitochondrial-enriched lysates or whole-cell extracts prepared under reducing conditions to detect the 14 kDa MRPS6 band.
• The antibody is also validated for ELISA and can be employed for detecting recombinant or native MRPS6 in sample matrices; optimal working dilutions should be determined empirically.
• As a polyclonal antibody, it may recognize multiple epitopes; include appropriate negative controls (e.g., knockdown/knockout cell lines or peptide blocking) to confirm specificity.
• Not tested for other applications such as IHC or IF by the manufacturer; if attempting these, thorough optimization is necessary.
• Shipment with ice packs; upon arrival, store at -20°C (or as detailed in the accompanying datasheet) for long-term stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is committed to providing a comprehensive supply channel for IVD raw materials, offering high-quality antibodies like this MRPS6 rabbit pAb to researchers and diagnostic developers. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary materials to support your projects from concept to clinic. With a focus on mitochondrial and ribosomal biology, we help streamline your sourcing needs, ensuring timely access to critical reagents for robust assay development.