Anti-MRPS35 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P82673

Core Product Specifications and Parameters

Biological Background: MRPS35 Function and Localization

• MRPS35 encodes a mitochondrial ribosomal protein that constitutes a component of the small (28S) subunit of the mitochondrial ribosome.
• As part of the mitoribosome, MRPS35 participates in the synthesis of 13 mitochondrial DNA-encoded polypeptides essential for oxidative phosphorylation.
• The protein is synthesized as a precursor with a mitochondrial transit peptide, which directs its import into the mitochondrion.
• Mature MRPS35 localizes to the mitochondrial matrix where it assembles into the small ribosomal subunit.
• MRPS35 has been identified in proteomics studies of the mitochondrial ribosome.
• The gene undergoes alternative splicing, producing multiple transcript variants.
• The 3D structure of the mitoribosome including MRPS35 has been determined, providing insights into its molecular architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length protein (amino acids 1-323), which may allow detection of multiple isoforms or splice variants.
• For Western blot, consider using mitochondrial-enriched lysates to enhance signal specificity.
• In IHC-P and IF/ICC applications, confirm mitochondrial localization by co-staining with organelle markers.
• Optimize antibody dilution and blocking conditions in your specific experimental system to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-MRPS35 product. For studies on mitochondrial ribosome function and protein synthesis, we also offer bulk ancillary reagents and can assist in sourcing rare-target raw materials. Partner with us to streamline your supply chain from discovery to clinical application.