Anti-MRPS31 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92665

Core Product Specifications and Parameters

Biological Background: MRPS31 Function and Localization

• MRPS31 (Mitochondrial Ribosomal Protein S31) is also known as S31mt, MRP-S31, and Imogen 38; it is encoded by the MRPS31 gene (Gene ID: 10240).
• It is a component of the small (28S) subunit of the mitochondrial ribosome, classified as small ribosomal subunit protein mS31, and is thought to participate in mitochondrial protein synthesis (UniProt: Q92665).
• The protein contains an N-terminal mitochondrial transit peptide, directing its import into the mitochondrion, where it functions as a ribonucleoprotein.
• UniProt classifies MRPS31 as a reference proteome member with proteomics identification and available 3D-structure data.
• The calculated molecular weight is ~45.3 kDa (45318 Da), consistent with the observed ~45 kDa band in Western blot assays.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 66–395, lacking the N-terminal transit peptide; thus, the antibody likely recognizes the mature mitochondrial form of MRPS31.
• For Western blot, prepare whole‑cell or mitochondrial‑enriched lysates from human or rat cells; expect a prominent band at approximately 45 kDa.
• In immunofluorescence/ICC, co‑stain with a mitochondrial marker (e.g., MitoTracker or anti‑TOM20) to confirm mitochondrial localization.
• ELISA conditions should be empirically optimized; recombinant MRPS31 protein or peptide may serve as a coating antigen.
• Batches are shipped on ice bags to maintain stability; aliquot and store appropriately upon receipt.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one‑stop sourcing experience for IVD raw materials. This anti‑MRPS31 rabbit polyclonal antibody is part of our extensive portfolio of optimized antibodies for mitochondrial biology applications. Beyond individual reagents, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare‑target raw materials—backed by technical consulting to support your project from concept to clinic.