Anti-MRPS31 Rabbit Monoclonal Antibody for WB, ELISA - Q92665

Core Product Specifications and Parameters

Biological Background: MRPS31 Function and Localization

• MRPS31 encodes the mitochondrial small ribosomal subunit protein mS31, also known as 28S ribosomal protein S31, mitochondrial (MRP-S31) or Imogen 38.
• This protein is a constituent of the mitochondrial ribosome (mitoribosome), a ribonucleoprotein complex dedicated to translating mitochondrial mRNA.
• UniProt annotation indicates localization to the mitochondrion, consistent with the presence of a transit peptide for mitochondrial import.
• It is listed in the human reference proteome and has been detected by proteomics methods, confirming its expression in human tissues.
• A 3D structure is available, demonstrating its structural role in the small subunit of the mitoribosome.
• The predicted molecular weight is 45318 Da, which matches the observed ~45 kDa band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 296-395 of human MRPS31; ensure your target protein isoform contains this C-terminal region, as splice variants lacking this segment may not be detected.
• For Western blot, mitochondrial-enriched lysates are recommended to increase sensitivity; a band near 45 kDa is expected, but confirm the exact molecular weight based on your gel system and loading controls.
• In ELISA, the antibody can be used for direct detection of recombinant MRPS31 or peptide antigens; titrate the antibody to determine optimal working concentration.
• Cross-reactivity with mouse and rat is documented, but sequence alignment of the immunogen region should be performed if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a complete selection of IVD raw materials, including validated monoclonal antibodies for mitochondrial targets like MRPS31. Our integrated platform streamlines procurement of bulk ancillary reagents, rare-target materials, and optimized antibody pairs, supporting your assay development pipeline. With a focus on mitochondrial biology and ribosomal research, CamelBio offers tailored technical consulting to help you transition from discovery to clinical application.