Anti-MRPS28 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y2Q9

Core Product Specifications and Parameters

Biological Background: MRPS28 Function and Localization

• MRPS28 (also known as MRPS35, S28mt, S35mt) encodes the mitochondrial 28S ribosomal protein bS1m, a component of the small subunit of the mitochondrial ribosome.
• As part of the mitoribosome small subunit, bS1m is involved in the translation of mitochondrial-encoded proteins essential for oxidative phosphorylation.
• MRPS28 is localized to the mitochondrion, consistent with its role in mitochondrial protein synthesis.
• The protein contains a transit peptide that directs its import into mitochondria.
• Post-translational modifications include acetylation and phosphorylation, which may regulate ribosome assembly or function.
• Mutations in MRPS28 are associated with primary mitochondrial disease, as indicated by the keyword 'Disease variant'.
• MRPS28 has been identified in proteomic studies as a reference proteome component and ribonucleoprotein complex member.
• The molecular weight of the processed protein is approximately 20.8 kDa (UniProt) or 21 kDa (as observed), consistent with the immunogen design.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-187) of human MRPS28; this region includes the transit peptide, so the antibody may recognize both precursor and mature forms in mitochondrial fractions.
• For Western blotting, optimize antibody dilution using appropriate loading controls and ensure efficient mitochondrial protein extraction and fractionation.
• In immunofluorescence/ICC, include mitochondrial markers (e.g., MitoTracker) to confirm colocalization and monitor potential cross-reactivity with cytoplasmic ribosomal proteins.
• The antibody is predicted to cross-react with mouse based on sequence homology; validation in mouse models is recommended for comparative studies.
• ELISA may require optimization of coating antigen and detection system; recombinant MRPS28 protein is suggested as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated rabbit polyclonal antibodies like this anti-MRPS28 product, to support mitochondrial research and translational studies. With expertise in antibody pair development, bulk reagent supply, and rare-target sourcing, CamelBio equips diagnostic manufacturers and laboratories with reliable, one-stop procurement solutions. Whether you need monoclonal/polyclonal antibodies, ancillary reagents, or technical consulting, we bridge the gap from concept to clinic.