Anti-MRPS26 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BYN8

Core Product Specifications and Parameters

Biological Background: MRPS26 Function and Localization

• MRPS26 (Mitochondrial Ribosomal Protein S26) is a component of the small (28S) subunit of the mitochondrial ribosome (mitoribosome), essential for mitochondrial protein synthesis.
• Also designated as MRP-S13 and MRP-S26, this protein belongs to the universal ribosomal protein family, contributing to the structural integrity of the ribosomal small subunit.
• Synthesized as a precursor with an N-terminal mitochondrial transit peptide that ensures its import into the organelle.
• Localizes exclusively to the mitochondrial matrix as part of the mature mitoribosome, where it facilitates the translation of 13 mitochondrially encoded mRNA species.
• The protein has a molecular weight of approximately 24 kDa (calculated 24212 Da) and has been identified in high-throughput proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen spans nearly full-length MRPS26 (aa 1-205), suggesting the polyclonal antibody may recognize multiple epitopes, potentially offering robust detection in denaturing WB and native applications.
• For WB, a band around 24 kDa is expected; consider using mitochondrial-enriched lysates to enhance signal.
• Cross-reactivity with mouse and rat MRPS26 (based on sequence homology) supports comparative studies; however, validate in each species' tissue context.
• In IHC-P, mitochondrial localization may yield punctate cytoplasmic staining; ensure adequate antigen retrieval and consider using a mitochondrial marker for colocalization.
• For ELISA, the antibody can be employed to detect recombinant or native MRPS26; titrate carefully to establish dynamic range.
• As a polyclonal antibody, lot-to-lot variability may occur; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, supporting mitochondrial and metabolic disease research with validated antibodies like this anti-MRPS26 polyclonal antibody. Beyond individual reagents, we offer integrated solutions including matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your diagnostic development from discovery to validation.