Anti-MRPS25 Rabbit Polyclonal Antibody for WB, ELISA - P82663

Core Product Specifications and Parameters

Biological Background: MRPS25 (Small ribosomal subunit protein mS25) Function and Localization

• MRPS25 encodes the mitochondrial small ribosomal subunit protein mS25, also known as 28S ribosomal protein S25, mitochondrial (MRP-S25, S25mt) and denoted by aliases RPMS25, COXPD50.
• It is an essential component of the mitochondrial ribosome (mitoribosome) dedicated to synthesizing proteins encoded by the mitochondrial genome.
• The protein is localized within the mitochondrion (SUBCELLULAR LOCATION: Mitochondrion).
• Alternative splicing generates multiple transcript variants of MRPS25.
• Mutations in MRPS25 are associated with primary mitochondrial disease, reflecting its critical role.
• It belongs to the ribosomal protein family and functions as a ribonucleoprotein complex.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 104–173 of human MRPS25; confirm that your detection system recognizes this epitope region.
• For western blotting (WB), the predicted molecular weight is approximately 20 kDa, so employ appropriate gel percentage and transfer conditions for low molecular weight proteins.
• In ELISA assays, consider using this polyclonal antibody for capture or detection, and validate with recombinant protein or cell lysates expressing endogenous MRPS25.
• Since this antibody is polyclonal and raised in rabbit, use appropriate anti‑rabbit secondary antibodies and account for potential batch‑to‑batch variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti‑MRPS25 polyclonal antibody supports mitochondrial biology and disease research, and we can further supply validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare target raw materials to streamline your assay development workflow.