Anti-MRPS23 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y3D9

Core Product Specifications and Parameters

Biological Background: MRPS23 Function and Localization

• MRPS23, also known as 28S ribosomal protein S23, mitochondrial (MRP-S23 or S23mt), is a mitochondrial ribosomal protein.
• It is a component of the small subunit (28S) of the mitochondrial ribosome, which is essential for mitochondrial protein synthesis.
• The gene maps to human chromosome 17 and encodes a protein with a predicted molecular weight of approximately 22 kDa (UniProt: 21771 Da).
• Subcellular localization: MRPS23 is located within the mitochondrion, consistent with its role in the organelle's translational machinery.
• Post-translational modifications include acetylation, as suggested by proteomics identification studies.
• Keywords associated with MRPS23: 3D-structure, acetylation, disease variant, mitochondrion, primary mitochondrial disease, proteomics identification, reference proteome, ribonucleoprotein, ribosomal protein.
• Disease associations: Variants in MRPS23 have been linked to primary mitochondrial disease and COXPD46 (combined oxidative phosphorylation deficiency 46).

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (aa 1-190) of human MRPS23; consider this when selecting peptide blocking or epitope mapping experiments.
• For Western blotting, a band around 22 kDa is expected; however, post-translational modifications or alternative splicing may result in additional bands. Optimization of lysate preparation and antibody concentration is recommended.
• In immunofluorescence/immunocytochemistry (IF/ICC), mitochondrial localization should be observed; co-staining with mitochondrial markers is advised for confirmation.
• ELISA can be used for quantitative detection; validate the antibody in your specific sample matrix and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including validated antibodies like this anti-MRPS23 polyclonal antibody. We support mitochondrial disease research and assay development with a comprehensive portfolio of antibody pairs, recombinant proteins, and ancillary reagents. For customized sourcing of rare targets, our team offers end-to-end procurement and technical consultation to accelerate your project from concept to clinic.