Anti-MRPS22 Rabbit Polyclonal Antibody for WB, ELISA - P82650

Core Product Specifications and Parameters

Biological Background: MRPS22 Function and Localization

• MRPS22, also known as 28S ribosomal protein S22, mitochondrial (MRP-S22, S22mt), is a component of the small (28S) subunit of the mitochondrial ribosome.
• Encoded by the MRPS22 gene (previously C3orf5 or RPMS22), this nuclear-encoded protein is imported into mitochondria to participate in mitochondrial protein synthesis.
• As a mitoribosomal protein, MRPS22 is expected to contribute to the structural integrity and function of the mitochondrial ribosome, which translates essential subunits of the oxidative phosphorylation complexes.
• Subcellularly, MRPS22 localizes to the mitochondrion, consistent with its function in the mitochondrial translation machinery.
• Post-translational modifications of MRPS22 include acetylation and phosphorylation, which may regulate ribosomal assembly or activity.
• Mutations in MRPS22 are associated with primary mitochondrial disease, highlighting its essential role in mitochondrial function.
• Alternative splicing of the MRPS22 gene produces multiple transcript variants, potentially modulating its function or expression.
• The protein has a molecular weight of approximately 41 kDa (calculated 41280 Da) and is a reference proteome member, widely studied in proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length (amino acids 1-360) of human MRPS22, which is likely to generate polyclonal antibodies recognizing multiple epitopes; users may consider epitope mapping if epitope information is needed.
• For Western blot (WB), a band at approximately 41 kDa is expected; validate in the relevant sample system, as cross-reactivity with human, mouse, and rat has been reported by the supplier.
• For ELISA applications, the recombinant protein used as immunogen can serve as a positive control; optimize coating and detection conditions as per standard protocols.
• Since MRPS22 is a mitochondrial protein, ensure proper sample preparation (e.g., mitochondrial enrichment) if low expression is a concern in whole-cell lysates.
• The antibody is supplied as a rabbit polyclonal; secondary anti-rabbit detection reagents should be used in WB and ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply one-stop IVD raw materials, facilitating development from concept to clinic. This anti-MRPS22 polyclonal antibody supports studies in mitochondrial biology and disease, and we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay and kit development. Our team brings expertise in molecular biology and immunodiagnostics to help bridge sourcing gaps for your most challenging targets.