Anti-MRPS2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y399

Core Product Specifications and Parameters

Biological Background: MRPS2 Function and Localization

• MRPS2, also designated S2mt, MRP-S2, or uS2m, encodes the mitochondrial 28S ribosomal protein S2, a core component of the small mitoribosomal subunit.
• It is indispensable for the assembly and structural integrity of the mitoribosome, and drives mitochondrial protein synthesis.
• The protein is strictly localized to the mitochondrial matrix.
• MRPS2 belongs to the evolutionarily conserved uS2 family of ribosomal proteins, underscoring its fundamental role in translation.
• High-resolution structural models (3D-structure) of the mitoribosome have mapped MRPS2 within the small subunit interface.
• Pathogenic variants in MRPS2 are linked to combined oxidative phosphorylation deficiency 36 (COXPD36), a primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant protein spanning the full length (aa 1–296) of human MRPS2, which may favor recognition of the native protein conformation as well as denatured forms.
• In Western blot (WB), a single band around 33 kDa is expected; consider using mitochondrial-enriched fractions to enhance signal intensity if expression is low.
• For immunohistochemistry (IHC-P), test a panel of mitochondrial-rich tissues (e.g., heart, skeletal muscle, kidney) and include appropriate blocking controls to mitigate non-specific background.
• ELISA can be employed for titer determination or quantitative detection of MRPS2 in biological fluids; cross-reactivity with the mouse ortholog enables its use in murine model analyses.
• As a polyclonal antibody, batch-to-batch consistency should be assessed; it is advisable to pilot-test each new lot in the intended assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for mitochondrial research and diagnostic development, offering this anti-MRPS2 polyclonal antibody alongside a wide portfolio of validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. Our network enables efficient sourcing of rare mitochondrial targets and supports your assay development from concept to clinical application.