Anti-MRPS18C Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y3D5

Core Product Specifications and Parameters

Biological Background: MRPS18C Function and Localization

• MRPS18C encodes a mitochondrial small ribosomal subunit protein, designated bS18m, which is an integral component of the 28S mitoribosome.
• Alternative names include 28S ribosomal protein S18-1, mitochondrial (MRP-S18-1), Small ribosomal subunit protein bS18c, and MRP-S18-c, highlighting its conserved role across species.
• The protein localizes exclusively to the mitochondrion, where it participates in the translation of mitochondrial-encoded proteins essential for oxidative phosphorylation.
• It features an N-terminal transit peptide that mediates its import into the mitochondrial matrix, after which it assembles into the small ribosomal subunit.
• A three-dimensional structure is available for this protein (UniProt keyword: 3D-structure), offering detailed insights into mitoribosome architecture and function.
• UniProt keywords classify MRPS18C as a ribonucleoprotein, ribosomal protein, and reference proteome entry, consistent with its structural and translational role in mitochondria.
• Proteomics identification confirms its presence in purified mitochondrial fractions, supporting its mitochondrial localization.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is approximately 16 kDa. Use reducing SDS-PAGE conditions and validate band specificity with appropriate positive and negative controls.
• In IHC-P and IF/ICC, strong mitochondrial staining is expected; consider co-staining with a mitochondrial marker (e.g., Tom20 or COX IV) to confirm subcellular specificity.
• For ELISA applications, the antibody can be utilized as either a capture or detection reagent; optimize coating concentrations and blocking conditions for your specific assay format.
• Due to the polyclonal nature, lot-to-lot variability may occur; it is advisable to validate each new lot in your intended application before scaling up.
• Reported cross-reactivity includes human, mouse, and rat; for other species, perform a sequence alignment to assess potential reactivity and consider testing in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated B2B IVD raw material supplier, CamelBio helps diagnostics manufacturers and research labs streamline procurement of validated antibodies and ancillary reagents. This anti-MRPS18C polyclonal antibody exemplifies our capability to supply high-quality reagents for mitochondrial research and related disease areas. Whether you need ready-to-use antibody pairs, bulk monoclonals, or custom sourcing for rare targets, CamelBio provides one-stop access to accelerate your assay development.