Anti-MRPS18A Rabbit Polyclonal Antibody for WB and ELISA - Q9NVS2

Core Product Specifications and Parameters

Biological Background: MRPS18A Function and Localization

• MRPS18A encodes the large ribosomal subunit protein mL66 (also known as 39S ribosomal protein S18-3, mitochondrial, MRP-S18-3, and MRP-S18-a), a component of the mitochondrial ribosome.
• This protein is part of the large subunit of the mitochondrial ribosome, contributing to mitochondrial protein synthesis.
• MRPS18A is located in the mitochondrion, consistent with its role in mitochondrial translation.
• The gene undergoes alternative splicing, potentially generating multiple transcript variants.
• Structural studies (3D-structure keyword) indicate that mL66 adopts a conserved ribosomal protein fold.
• It belongs to the ribosomal protein bS18a family and is a ribonucleoprotein.
• The protein has a molecular weight of approximately 22 kDa (calculated 22,184 Da) and contains a transit peptide for mitochondrial import.
• Keywords from UniProt include: 3D-structure, Alternative splicing, Mitochondrion, Proteomics identification, Reference proteome, Ribonucleoprotein, Ribosomal protein, Transit peptide.

Experimental Guidance and Technical Tips

• The immunogen used was a synthetic peptide corresponding to amino acids 97-196 of human MRPS18A (NP_060605.1). For custom antibody development, this region could be considered as an epitope candidate.
• The antibody is expected to detect a band around 22 kDa in Western blotting. Ensure appropriate gel percentage and transfer conditions for small proteins.
• This antibody shows cross-reactivity with human and mouse samples; it may also react with other species based on sequence homology. Validate in the relevant sample system.
• For ELISA, consider coating with the peptide or recombinant protein and optimizing antibody concentration.
• As a polyclonal antibody, lot-to-lot variability may occur; always titrate and validate for each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers and research institutions. This anti-MRPS18A antibody supports studies of mitochondrial ribosome function and its implications in disease. Beyond antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development from concept to clinic.