Anti-MRPS15 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P82914

Core Product Specifications and Parameters

Biological Background: MRPS15 Function and Localization

• MRPS15 (also known as uS15m, MRP-S15, S15mt, DC37) is a mitochondrial ribosomal protein that constitutes part of the 28S small subunit.
• The protein is encoded by the MRPS15 gene (alternative name RPMS15) and is synthesized with an N-terminal mitochondrial transit peptide for import into the mitochondrial matrix.
• Within the mitochondrial ribosome, MRPS15 participates in the translation of mtDNA-encoded mRNAs, contributing to mitochondrial protein synthesis.
• Keywords associated with MRPS15 include Ribonucleoprotein, Ribosomal protein, and Transit peptide, reflecting its RNA-binding role and mitochondrial targeting.
• The protein has been identified in high-throughput proteomics studies, confirming its expression in human cells.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to residues 158-257 of human MRPS15, the antibody is expected to recognize an epitope within this region.
• For Western blot (WB), a band at ~30 kDa should be detected in human mitochondrial or whole-cell lysates; optimize loading and transfer conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), standard fixation and antigen retrieval methods may be employed; verify mitochondrial staining pattern.
• For ELISA assays, perform titration experiments to determine optimal antibody dilution for capture or detection.
• The antibody is reported to be specific for human MRPS15; cross-reactivity with other species or related proteins should be experimentally validated if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-MRPS15 clone. For mitochondrial biology and protein synthesis research, we offer optimized antibody pairs, bulk ancillary reagents, and flexible sourcing for rare targets. Contact us to streamline your supply chain from concept to clinic.