Anti-MRPS14 Rabbit Polyclonal Antibody for WB, ELISA - O60783

Core Product Specifications and Parameters

Biological Background: MRPS14 Function and Localization

• MRPS14 (mitochondrial ribosomal protein S14) is a component of the small (28S) subunit of the mitochondrial ribosome.
• It plays a critical role in mitochondrial protein synthesis, specifically in translating mitochondrially encoded subunits of the oxidative phosphorylation complexes.
• The protein is localized to the mitochondrion.
• Mutations in MRPS14 have been associated with primary mitochondrial diseases, such as combined oxidative phosphorylation deficiency 38 (COXPD38).
• It is referred to by several aliases, including S14mt, MRP-S14, and uS14m.
• The molecular weight of the mature protein is approximately 15 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (1-128), which may include conserved domains; thus, cross-reactivity to mouse and rat is expected.
• For Western blot, a band at approximately 15 kDa is expected; consider using a mitochondrial enrichment protocol or whole cell lysates from human, mouse, or rat tissues.
• For ELISA, the antibody can be used as both capture and detection reagent in sandwich assays, but optimal concentrations should be determined experimentally.
• As a polyclonal antibody, batch-to-batch variability may exist; validate in your specific assay system.
• The antibody may recognize both the precursor and mature forms; verify localization by mitochondrial fractionation if needed.
• Use appropriate controls (e.g., MRPS14 knockdown or knockout lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for mitochondrial disease research. As your one-stop sourcing partner, we can also assist in sourcing rare targets like MRPS14 and provide technical support from concept to clinic. Contact us for bulk quantities or customized solutions tailored to your diagnostic development needs.