Anti-MRPS12 Rabbit Polyclonal Antibody for WB, ELISA - O15235

Core Product Specifications and Parameters

Biological Background: MRPS12 Function and Localization

• MRPS12 encodes the mitochondrial 28S ribosomal protein S12 (MRP-S12), a component of the small ribosomal subunit in mitochondria.
• It is known as uS12m in the universal ribosomal protein nomenclature and plays a critical role in mitochondrial protein translation.
• The gene is also designated as RPMS12, RPSM12, and MT-RPS12.
• The MRPS12 protein localizes exclusively to the mitochondrion, consistent with its role in mitochondrial ribosomes.
• It is a ribonucleoprotein that, together with mitochondrial rRNA and other proteins, forms the functional small subunit (28S) of the mitochondrial ribosome.
• The mature protein has a calculated molecular weight of approximately 15 kDa (15173 Da based on UniProt), although observed molecular weight may vary due to post-translational modifications.
• UniProt keywords include transit peptide, reflecting its mitochondrial targeting sequence, and 3D-structure, indicating structural characterization has been performed.

Experimental Guidance and Technical Tips

• Given that the immunogen is derived from human CYB5R3 rather than MRPS12, we recommend thorough validation of binding specificity to the MRPS12 target in your sample system using appropriate controls (e.g., knockdown or overexpression) before relying on this antibody for MRPS12 detection.
• For Western blotting, a band at ~15 kDa is expected for MRPS12. Optimize sample preparation to preserve mitochondrial proteins, as MRPS12 may be lost during standard cytosolic extraction.
• For ELISA, ensure the antigen coating and detection conditions are optimized for the polyclonal nature of the antibody, and include proper blocking steps to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including antibodies for mitochondrial biology targets like MRPS12. We offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Our sourcing expertise extends to rare and custom targets, ensuring you have the critical reagents needed from concept to clinic.