Anti-MRPS12 Rabbit Polyclonal Antibody for WB, ELISA - O15235

Core Product Specifications and Parameters

Biological Background: MRPS12 Function and Localization

• MRPS12 encodes the human mitochondrial ribosomal protein S12, also known as uS12m, a component of the small 28S subunit of the mitochondrial ribosome.
• As a mitochondrial ribosomal protein, MRPS12 is essential for mitochondrial protein synthesis, contributing to the translation of the 13 mitochondrial DNA-encoded oxidative phosphorylation subunits.
• The protein localizes exclusively to the mitochondrion, where it assembles into the small ribosomal subunit after import; it contains a transit peptide targeting it to the mitochondria.
• Its molecular weight is approximately 15.2 kDa (calculated) and migrates at ~15 kDa in SDS-PAGE.
• MRPS12 is part of the ribosomal protein uS12 family, conserved across bacteria, mitochondria, and eukaryotes, though it specifically functions in mitochondrial ribosomes.
• Structural studies (keyword: 3D-structure) have elucidated its position within the mitochondrial ribosome, revealing critical interactions with rRNA and other ribosomal proteins.
• The gene is designated MRPS12 and is also known by aliases such as RPMS12, RPSM12, MT-RPS12, RPS12, and MPR-S12.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30-138 of human MRPS12 (NP_203527.1), a region predicted to be surface-exposed in the mature protein, making it suitable for detecting endogenous MRPS12.
• This antibody is recommended for Western blot (WB) and ELISA applications; for WB, validate the antibody's specificity in relevant human cell or tissue lysates with a band expected at ~15 kDa.
• Since MRPS12 is a mitochondrial protein, ensure proper mitochondrial enrichment or total cell lysis under denaturing conditions to solubilize membrane-associated complexes.
• Consider the possibility of cross-reactivity with other species, but the antibody has been validated for human cross-reactivity only, as indicated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering high-quality IVD raw materials such as this anti-MRPS12 antibody for mitochondrial research. Beyond primary antibodies, our portfolio includes validated antibody pairs, bulk ancillary reagents, and customized sourcing of rare-target biological reagents to streamline your assay development from concept to clinic. Partner with us to efficiently bridge your raw material needs while accelerating your diagnostic progress.