Anti-MRPS11 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P82912

Core Product Specifications and Parameters

Biological Background: MRPS11 Function and Localization

• Small ribosomal subunit protein uS11m, also known as 28S ribosomal protein S11, mitochondrial (MRP-S11, S11mt) and cervical cancer proto-oncogene 2 protein (HCC-2).
• A component of the mitochondrial 28S ribosomal small subunit, essential for mitochondrial protein synthesis.
• Encoded by the nuclear gene MRPS11, and imported into mitochondria via an N-terminal transit peptide.
• Localized to the mitochondrial matrix, where it participates in the translation of mitochondrial DNA-encoded proteins.
• Alternative splicing generates multiple isoforms, with molecular weights around 16 kDa and 20 kDa.
• Keywords suggest involvement in proteomics identification, reference proteome, and ribonucleoprotein complex formation.
• May have a role in cervical cancer as indicated by its alias HCC-2 (cervical cancer proto-oncogene 2).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 95-194) of human MRPS11, suitable for recognizing multiple isoforms.
• For Western blot (WB), test both reducing and non-reducing conditions to optimize detection of the 16–20 kDa bands.
• In immunofluorescence (IF/ICC), anticipate mitochondrial staining; co-staining with mitochondrial markers is recommended.
• For ELISA applications, the peptide may serve as a positive control; validate in the relevant sample system.
• Cross-reactivity with mouse and rat orthologs has been tested, but confirm specificity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with global manufacturers to provide a comprehensive range of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for mitochondrial and cancer research. This anti-MRPS11 antibody is part of our portfolio supporting mitochondrial biology, from ribosomal protein studies to proto-oncogene investigation. We offer one-stop sourcing for diagnostic developers, with supply chain solutions that streamline procurement from concept to clinic.