Anti-MRPS11 Monoclonal Antibody for WB, IF-P, ELISA - P82912

Core Product Specifications and Parameters

Biological Background: MRPS11 Function and Localization

• MRPS11 encodes the small ribosomal subunit protein uS11m, a key component of the mitochondrial ribosome (mitoribosome).
• Known aliases include MRP-S11, S11mt, and cervical cancer proto-oncogene 2 protein (HCC-2).
• The protein is exclusively localized to the mitochondrion, consistent with its transit peptide and subcellular annotation.
• As a ribosomal protein, uS11m participates in mitochondrial protein synthesis, which is critical for oxidative phosphorylation.
• Alternative splicing of the MRPS11 gene generates multiple transcript variants.
• The mature protein has a calculated molecular mass of approximately 20.6 kDa, though it is commonly detected at ~21 kDa in western blot applications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the central region (amino acids 95–194) of human MRPS11; verify epitope accessibility in your specific sample type.
• For western blotting, a band near 21 kDa is expected in mitochondrial fractions from human, mouse, and rat samples; include appropriate mitochondrial markers as loading controls.
• For immunofluorescence on paraffin‑embedded sections (IF-P), optimize antigen retrieval and antibody dilution, as endogenous MRPS11 levels may be low.
• In ELISA, confirm optimal coating conditions and the compatibility of your secondary detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For mitochondrial and ribosomal protein targets like MRPS11, we offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents to streamline your assay development. Leverage our global sourcing network for rapid access to even rare‑target raw materials.