Anti-MRPL9 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BYD2

Core Product Specifications and Parameters

Biological Background: MRPL9 Function and Localization

• MRPL9 encodes the large ribosomal subunit protein bL9m, a component of the mitochondrial 39S ribosomal subunit.
• As part of the mitochondrial ribosome, MRPL9 is essential for the translation of 13 mitochondrial DNA-encoded proteins critical for oxidative phosphorylation.
• The protein localizes strictly to the mitochondrion, mediated by an N-terminal transit peptide that is cleaved upon import.
• MRPL9 belongs to the ribosomal protein bL9 family and is ubiquitously expressed, reflecting the fundamental requirement for mitochondrial translation.
• Keywords such as Ribonucleoprotein, Ribosomal protein, and Mitochondrion highlight its role in mitochondrial protein synthesis.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 30 kDa; include a protein ladder spanning 15–70 kDa for accurate sizing.
• When performing immunofluorescence, co-stain with a mitochondrial marker (e.g., MitoTracker™ or anti-TOMM20) to verify mitochondrial localization.
• The immunogen (aa 53–267) targets a central region conserved in human, mouse, and rat, but validate cross-reactivity in your experimental conditions.
• For ELISA applications, titrate the antibody to determine optimal coating and detection concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between research discovery and diagnostic development by providing comprehensive access to high-quality IVD raw materials, including this anti-MRPL9 antibody. With a focus on mitochondrial targets and ribosomal proteins, we supply validated antibody pairs, bulk reagents, and rare target sourcing to support your pipeline from concept to clinic. Partner with us for seamless, one-stop procurement tailored to your project needs.