Anti-Mrpl55 Polyclonal Antibody for WB and ELISA - Q9CZ83

Core Product Specifications and Parameters

Biological Background: Mrpl55 Function and Localization

• Mrpl55 encodes the mitochondrial large ribosomal subunit protein mL55 (also known as 39S ribosomal protein L55, L55mt, or MRP-L55), a component of the mitochondrial ribosome.
• As a structural ribosomal protein, it participates in the assembly and function of the mitochondrial large subunit, essential for mitochondrial protein synthesis.
• The protein contains a transit peptide that directs its import into the mitochondrion, where it localizes to the mitochondrial matrix.
• Alternative splicing of the Mrpl55 gene generates multiple transcript variants, potentially modulating its function or regulation.
• Post-translational modifications include phosphorylation, which may influence ribosomal activity or interactions.
• The mature protein has a molecular weight of approximately 15 kDa, consistent with its role as a ribosomal protein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 33-127 of mouse Mrpl55, which excludes the predicted transit peptide, ensuring recognition of the mature mitochondrial form.
• For Western blot, a band at ~15 kDa is expected; consider using a high-percentage gel (e.g., 12-15%) for better resolution of low molecular weight proteins.
• Since this is a polyclonal antibody, lot-to-lot variability may occur; validate each new lot in your specific experimental system.
• Cross-reactivity has been reported for human and mouse; for other species, perform sequence alignment and preliminary testing.
• This antibody is suitable for ELISA applications; optimal dilution should be determined empirically.
• For mitochondrial protein extraction, ensure effective lysis and consider mitochondrial enrichment protocols to enhance signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with reliable access to critical reagents. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-Mrpl55 product, and bulk ancillary reagents for mitochondrial research and protein synthesis studies. We also specialize in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.