Anti-MRPL54 Polyclonal Antibody for WB, IHC-P, ELISA - Q6P161

Core Product Specifications and Parameters

Biological Background: MRPL54 Function and Localization

• MRPL54 encodes the mitochondrial large ribosomal subunit protein mL54, also known as 39S ribosomal protein L54, mitochondrial; L54mt; and MRP-L54.
• It is a component of the mitochondrial ribosome, which mediates protein synthesis within the organelle, as indicated by its classification under Ribonucleoprotein and Ribosomal protein keywords.
• The protein is localized to the mitochondrion, consistent with its role in mitochondrial translation, and possesses a transit peptide for mitochondrial import.
• MRPL54 is part of the human reference proteome and has an experimentally determined 3D structure, pointing to conserved structural features.
• The calculated molecular weight is 15,819 daltons, aligning with the observed band at ~16 kDa in denaturing electrophoresis.

Experimental Guidance and Technical Tips

• For Western blotting, load 20–30 µg of mitochondrial-enriched lysate and probe with the antibody; the expected band is approximately 16 kDa.
• In IHC-P, test on formalin-fixed paraffin-embedded human tissue sections; antigen retrieval with citrate buffer (pH 6.0) may improve staining.
• For ELISA, consider using the antibody as a capture or detection reagent, but preliminary checkerboard titrations are recommended to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop procurement partner for IVD raw materials, providing monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents. Our portfolio supports mitochondrial and ribosomal protein research, enabling diagnostic manufacturers and laboratories to accelerate assay development. For specialized targets like MRPL54, we offer validated antibody pairs and custom sourcing to meet your project needs.