Anti-MRPL52 (KD Validated) Rabbit Polyclonal Antibody for WB and ELISA - Q86TS9

Core Product Specifications and Parameters

Biological Background: MRPL52 Function and Localization

• MRPL52 is a component of the mitochondrial large ribosomal subunit (39S), also known as mL52, L52mt, or MRP-L52. It belongs to the ribosomal protein L52 family.
• The protein contains a transit peptide that directs its import into mitochondria, where it participates in mitochondrial protein synthesis as a structural constituent of the mitoribosome.
• Alternative splicing of the MRPL52 gene generates multiple transcript variants, potentially contributing to functional diversity in mitochondrial ribosomes.
• MRPL52 is classified as both a ribosomal protein and a ribonucleoprotein, highlighting its essential role in the assembly and function of the mitochondrial translation machinery.
• The protein exhibits a molecular weight of approximately 14 kDa (precursor 13.7 kDa), consistent with its detection near 14 kDa in SDS-PAGE and western blot analysis.
• The 3D structure of the human mitochondrial ribosome, including MRPL52, has been resolved, providing insights into its spatial organization and interaction network.
• MRPL52 has been identified in proteomics studies, confirming its endogenous expression in human cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 24–123 of human MRPL52, targeting a region likely conserved in the mature processed protein; confirm epitope accessibility under native and denaturing conditions.
• For western blot, a single band near 14 kDa is expected; optimize primary antibody dilution and consider using mitochondrial‑enriched lysates to enhance signal.
• In ELISA, both direct antigen coating (recombinant protein/peptide) and sandwich formats may be evaluated; assay conditions should be empirically determined.
• As a polyclonal antibody, lot‑to‑lot consistency should be verified through control experiments; include a positive control (e.g., HeLa or HEK293T whole cell lysate).
• Cross‑reactivity has been demonstrated for human; reactivity with other species has not been tested and should be validated prior to use in non‑human model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For mitochondrial biology and ribosomal protein research, we offer high-quality reagents like this anti‑MRPL52 antibody to support diagnostic development. Our one‑stop sourcing services cover rare target reagent procurement, bulk supply, and technical consulting—streamlining your path from concept to clinic.