Anti-MRPL50 Polyclonal Antibody for WB, ELISA - Q8N5N7

Core Product Specifications and Parameters

Biological Background: MRPL50 Function and Localization

• MRPL50 encodes the large ribosomal subunit protein mL50, a component of the mitochondrial ribosome (mitoribosome). It is also designated 39S ribosomal protein L50, mitochondrial (L50mt or MRP-L50).
• The gene produces multiple transcript variants via alternative splicing, potentially yielding distinct protein isoforms.
• mL50 localizes to the mitochondrion, where it plays a critical role in the translation of mitochondrial-encoded mRNAs.
• As a ribosomal protein of the large subunit, mL50 participates in the assembly and structural integrity of the mitoribosome, facilitating peptide bond formation during mitochondrial protein synthesis.
• The protein has been identified through high-throughput proteomic analyses, confirming its expression in human cell lines.
• According to UniProt, MRPL50 is classified under keywords including Ribonucleoprotein, Ribosomal protein, Mitochondrion, and Alternative splicing, highlighting its functional context.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (aa 1-100) of human MRPL50. This design suggests the antibody may recognize linear epitopes; confirm specificity in your sample system using appropriate controls (e.g., knockout or overexpression cell lysates).
• For Western blot (WB), the calculated molecular weight of the target protein is ~18 kDa (UniProt: 18.3 kDa). Ensure appropriate gel percentage and transfer conditions to resolve this low-molecular-weight protein.
• In ELISA applications, consider a direct or indirect ELISA format with proper blocking and detection steps to minimize background, especially when working with complex sample matrices.
• Since this is a polyclonal antibody, lot-to-lot variability can occur. Validate each new lot in your assay system and consider using a qualified secondary antibody for consistent performance.
• As the protein is mitochondrial, sample preparation for WB may require efficient lysis and possibly mitochondrial enrichment to achieve detectable signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with reliable access to antibodies, recombinant proteins, and ancillary reagents. This anti-MRPL50 polyclonal antibody is designed for studies involving mitochondrial ribosome function and protein synthesis, supporting research in mitochondrial biology and related disorders. Beyond individual catalog items, CamelBio offers customizable sourcing solutions for rare targets, bulk orders, and validated antibody pairs—streamlining your path from concept to clinical application.