Anti-MRPL46 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H2W6

Core Product Specifications and Parameters

Biological Background: MRPL46 Function and Localization

• MRPL46 is a mitochondrial ribosomal protein that forms part of the large (39S) subunit of the mitoribosome, as indicated by its classification as a ribonucleoprotein and ribosomal protein (UniProt Keywords).
• The protein is synthesized as a precursor with a mitochondrial transit peptide and is imported into the mitochondrial matrix, where it assembles into the large ribosomal subunit (SUBCELLULAR LOCATION and Transit peptide keyword).
• As a component of the mitochondrial ribosome, MRPL46 is expected to contribute to the translation of mitochondrial-encoded mRNAs, producing proteins essential for oxidative phosphorylation.
• MRPL46 undergoes post-translational acetylation, as identified by mass spectrometry-based proteomics (keyword: Acetylation).
• The 3D structure of the human mitochondrial ribosome, including MRPL46, has been solved, providing insights into its role in mitoribosome architecture (keyword: 3D-structure).
• Alternative names include 39S ribosomal protein L46, mitochondrial (L46mt, MRP-L46), as well as P2ECSL and LIECG2.
• The gene MRPL46 (formerly C15orf4) is located on chromosome 15 and is widely expressed as part of the mitochondrial translation machinery.

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to the expected molecular weight of approximately 32 kDa should be detected in samples rich in mitochondria (e.g., heart, skeletal muscle, or cell lines). Consider using mitochondrial fractionation to enrich the target.
• In immunohistochemistry (IHC-P), appropriate antigen retrieval and detection conditions should be optimized; co-localization with a mitochondrial marker is recommended to confirm specificity.
• For ELISA applications, the antibody can be used as a capture or detection reagent, but proper cross-reactivity testing against closely related ribosomal proteins is advised given the polyclonal nature.
• The immunogen spans the full-length protein (aa 1-279), so the antibody may recognize multiple epitopes; non-specific bands may be minimized by adjusting blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting mitochondrial research and diagnostic development. For MRPL46 and other mitochondrial targets, we provide validated antibody pairs, bulk quantities, and ancillary reagents to streamline assay design and manufacturing. Our integrated supply chain ensures reliable, quality-controlled raw materials for your diagnostic needs.