Anti-MRPL43 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N983

Core Product Specifications and Parameters

Biological Background: MRPL43 Function and Localization

• MRPL43 encodes the mitochondrial large ribosomal subunit protein mL43 (also known as 39S ribosomal protein L43, mitochondrial, L43mt, MRP-L43, or bMRP36a).
• As a component of the 39S mitochondrial ribosome, mL43 participates in the translation of the 13 mitochondrially-encoded mRNAs that are essential for oxidative phosphorylation.
• The protein is synthesized as a precursor with an N-terminal mitochondrial transit peptide, which is cleaved upon import into the mitochondrial matrix.
• Subcellular localization is exclusively mitochondrial, consistent with its role in mitochondrial protein synthesis.
• Tissue expression analysis reveals highest levels in skeletal muscle and testis, with lower expression in heart, brain, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. MRPL43 expression is co-regulated with TWNK (Twinkle), a mitochondrial DNA helicase.
• Alternative splicing of the MRPL43 gene produces multiple variants, potentially modulating ribosome assembly or function.
• Structural studies have resolved the 3D structure of human mitochondrial ribosome, including mL43's position near the polypeptide exit tunnel.
• The protein is widely identified in proteomics screens and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 80-155 of human MRPL43, which corresponds to a central region of the protein. This region is conserved in mouse and rat, accounting for the predicted cross-reactivity.
• For Western blot, expect a band at approximately 23 kDa in mitochondrial-enriched lysates. Use appropriate loading controls for mitochondrial proteins.
• In immunofluorescence/ICC, ensure permeabilization protocols that allow antibody access to mitochondrial compartments. Co-staining with a mitochondrial marker (e.g., MitoTracker) is recommended to confirm specificity.
• For ELISA, the polyclonal antibody can be used as a capture or detection reagent; however, optimal dilutions must be determined empirically.
• As a polyclonal antibody, lot-to-lot consistency may vary; consider validating each new lot in your assay system.
• Cross-reactivity with human, mouse, and rat suggests suitability for studies in rodent models, but verify reactivity with your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, technical services, and development consulting, supporting every stage from concept to clinic. For mitochondrial research targets like MRPL43, we offer validated polyclonal antibodies alongside custom sourcing of rare targets, bulk ancillary reagents, and expert assistance in assay development. Our integrated supply chain ensures reliable access to high-quality materials for diagnostic manufacturers and research laboratories.