Anti-MRPL42 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y6G3

Core Product Specifications and Parameters

Biological Background: MRPL42 Function and Localization

• MRPL42 (also known as L31mt, L42mt, MRP-L31, MRP-L42, and MRP-S32) is a component of the mitochondrial large ribosomal subunit, essential for mitochondrial protein synthesis.
• As a mitochondrial ribosomal protein, MRPL42 participates in the translation of 13 mitochondrial DNA-encoded mRNAs that are critical for oxidative phosphorylation.
• The protein is synthesized in the cytoplasm and imported into the mitochondrial matrix; it contains a transit peptide at the N-terminus that directs its mitochondrial localization.
• MRPL42 is strictly localized to the mitochondrion, where it assembles into the 39S large subunit of the mitochondrial ribosome.
• Based on proteomic studies, MRPL42 has been identified as a mitochondrial protein, and its 3D structure has been determined, providing insights into its ribosomal interactions.
• Keywords from UniProt include Mitochondrion, Ribonucleoprotein, Ribosomal protein, and Transit peptide, underscoring its mitochondrial and translational roles.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human MRPL42 protein (amino acids 1-142), suggesting that the antibody recognizes native and denatured forms in applications such as WB and ELISA.
• For Western blot analysis, a band around 17 kDa is expected in human and mouse samples; consider using mitochondrial-enriched lysates to improve detection sensitivity.
• Because this is a polyclonal antibody, lot-to-lot variability is possible; validate new lots in your specific assay system before large-scale use.
• Optimal antibody dilutions should be determined experimentally for each application; typical starting points might be 1:500-1:2000 for WB and 1:100-1:1000 for ELISA, but empirical testing is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. Our MRPL42 rabbit polyclonal antibody supports mitochondrial research and related IVD assay development. For bulk orders or custom sourcing of other rare-target raw materials, please contact our team to discuss your specific requirements.