Anti-MRPL40 Polyclonal Antibody for WB, ELISA - Q9NQ50

Core Product Specifications and Parameters

Biological Background: MRPL40 Function and Localization

• The MRPL40 gene encodes the mitochondrial large ribosomal subunit protein mL40 (also known as 39S ribosomal protein L40, MRP-L40, L40mt), a component of the mitoribosome.
• As part of the large subunit, mL40 contributes to mitochondrial translation machinery essential for synthesis of mitochondrial-encoded proteins.
• Alternative names URIM (up-regulated in metastasis) and NLVCF (nuclear localization signal-containing protein deleted in velocardiofacial syndrome) hint at extra-ribosomal roles or genomic context; the gene is located in the 22q11.2 region associated with velocardiofacial syndrome.
• The protein contains a transit peptide that directs its import into mitochondria, where it localizes.
• MRPL40 is ubiquitously expressed in human tissues.
• UniProt keywords: 3D-structure (indicating structural studies have been performed), ribonucleoprotein, ribosomal protein, reference proteome, transit peptide.

Experimental Guidance and Technical Tips

• For Western blot, use reducing SDS-PAGE and appropriate human cell lysates (e.g., HeLa, HEK293) given ubiquitous expression; the predicted molecular weight is ~24 kDa, though migrating bands may vary.
• For ELISA, the antibody can be used against recombinant MRPL40 protein or cell/tissue extracts; validate coating and detection conditions.
• The immunogen (aa 45-206 of human MRPL40) corresponds to the mature processed protein, so reactivity is expected against endogenous human MRPL40.
• To confirm specificity, consider using a blocking peptide or siRNA-mediated knockdown of MRPL40 in cell models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and research institutes with validated antibodies, bulk reagents, and technical support from concept to clinic. Our portfolio of mitochondrial research tools, including anti-MRPL40 antibodies like this one, supports studies in mitochondrial dysfunction, cancer metabolism, and genetic disorders. Whether you need optimized antibody pairs, large-scale production, or rare target sourcing, CamelBio streamlines your supply chain with quality-assured materials.