Anti-MRPL38 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96DV4

Core Product Specifications and Parameters

Biological Background: MRPL38 Function and Localization

• MRPL38 encodes the mitochondrial large ribosomal subunit protein mL38, also known as 39S ribosomal protein L38, mitochondrial (L38mt/MRP-L38) (UniProt Q96DV4).
• The protein contains a coiled coil domain and a mitochondrial transit peptide, facilitating its import into mitochondria and assembly into the large subunit (UniProt Q96DV4).
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversification (UniProt Q96DV4).
• It localizes exclusively to the mitochondrion, where it participates in mitochondrial protein translation as an integral component of the 39S ribosomal subunit (UniProt Q96DV4).
• Orthologs are conserved across mammals, including mouse and rat, indicating a fundamental role in mitochondrial ribosomal integrity (UniProt Q96DV4).
• Structural studies provide 3D structural insights into its arrangement within the mitoribosome (UniProt Q96DV4).
• Proteomics-based identification has confirmed its presence in purified human mitochondrial ribosome preparations (UniProt Q96DV4).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 35–380) excludes the N-terminal transit peptide, suggesting the antibody recognizes the mature mitochondrial protein.
• For Western blot, the predicted molecular weight is ~45 kDa (UniProt: 44.6 kDa); mitochondrial enrichment or subcellular fractionation may enhance detection sensitivity.
• In IHC-P, optimize antigen retrieval protocols for mitochondrial targets; paraformaldehyde-fixed tissue sections are suitable for further validation.
• Cross-reactivity with mouse and rat MRPL38 is supported by sequence homology; antibody performance should be verified in the relevant species and tissue context.
• For ELISA applications, consider using recombinant full-length or fragment proteins as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw-material procurement, offering this anti-MRPL38 polyclonal antibody alongside a comprehensive range of reagents for mitochondrial biology research. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or support sourcing rare-target raw materials, we streamline your assay development workflow from concept to clinic. Explore our catalog or contact our technical team for customized solutions tailored to your diagnostic and research needs.