Anti-MRPL36 Rabbit Polyclonal Antibody for WB and ELISA - Q9P0J6

Core Product Specifications and Parameters

Biological Background: MRPL36 Function and Localization

• MRPL36 (also known as 39S ribosomal protein L36, mitochondrial, L36mt, MRP-L36, or bL36m) is a core structural component of the large mitochondrial ribosomal subunit.
• Encoded by the MRPL36 gene (designated BRIP1 in some contexts) in humans, it plays a crucial role in mitochondrial protein synthesis and energy metabolism.
• The protein belongs to the ribosomal bL36m family and is highly conserved across eukaryotic species, reflecting its fundamental role in mitoribosome assembly.
• MRPL36 is exclusively localized to the mitochondrion, as supported by its N-terminal transit peptide and proteomic identification.
• Based on available 3D-structural data, it integrates into the central protuberance of the large mitoribosomal subunit, contributing to tRNA binding and translation fidelity.
• Its alternative name “BRCA1-interacting protein 1” suggests a putative nuclear function in DNA repair, but its primary residence and established function remain mitochondrial.
• Multiple proteomics studies have confirmed the presence and interactions of MRPL36 within the mitochondrial ribosomal complex.
• Keywords associated with this protein include 3D-structure, mitochondrion, ribonucleoprotein, ribosomal protein, transit peptide, emphasizing its structural role in translation.

Experimental Guidance and Technical Tips

• For Western blotting, a 12 kDa band is expected in human mitochondrial fractions; consider using a 12% or higher Tris-glycine gel and appropriate membrane for low-molecular-weight protein transfer.
• Given the human-specific cross-reactivity, validate the antibody with a panel of relevant human cell lines or tissue lysates.
• In ELISA, using the recombinant immunogen (amino acids 39–103) as a positive control can help optimize conditions and confirm reactivity.
• The immunogen spans a conserved central region; verify that the epitope is accessible in native versus denatured conditions depending on your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This MRPL36 rabbit polyclonal antibody, along with our portfolio of validated antibodies, recombinant proteins, and ancillary reagents, empowers your mitochondrial biology and translational research workflows. Whether you need optimized antibody pairs, bulk monoclonal/polyclonal supplies, or rare target sourcing, CamelBio delivers reliable, high-quality solutions to accelerate your projects.