Anti-MRPL33 Rabbit Polyclonal Antibody for IHC-P, ELISA - O75394

Core Product Specifications and Parameters

Biological Background: MRPL33 Function and Localization

• MRPL33 encodes the 39S ribosomal protein bL33m (also known as L33mt or MRP-L33), a structural component of the mitochondrial large ribosomal subunit.
• It belongs to the universal ribosomal protein L33 family and is essential for mitochondrial translation and oxidative phosphorylation system biogenesis.
• The protein localizes to the mitochondrial matrix following import via an N-terminal transit peptide.
• Alternative splicing of the MRPL33 gene generates multiple transcript variants, potentially giving rise to distinct isoforms.
• Keywords highlight its roles as a ribonucleoprotein and ribosomal protein, with available 3D-structure and proteomics identification data.
• A transit peptide sequence ensures proper targeting to the mitochondrion, and its small molecular weight (~7.6 kDa) is typical of ribosomal proteins.

Experimental Guidance and Technical Tips

• The immunogen consists of the N-terminal 65 amino acids of human MRPL33 (NP_004882.1); verify reactivity against isoforms if using samples with alternative splicing.
• For IHC-P, optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0) and include appropriate positive (e.g., human heart or skeletal muscle) and negative controls.
• In ELISA applications, consider the polyclonal nature and potential batch variability; validate with recombinant MRPL33 or mitochondrial lysates.
• Due to the low molecular weight of MRPL33 (7 kDa), use high-percentage SDS-PAGE gels or gradient gels for western blot detection if adapting this antibody for research use (note: application not specifically listed as WB).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, connecting diagnostic developers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For mitochondrial research and ribosomal protein targets like MRPL33, CamelBio can assist in procuring rare-target raw materials and offering technical consulting to streamline your assay development from concept to commercialization.