Anti-MRPL30 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8TCC3

Core Product Specifications and Parameters

Biological Background: MRPL30 Function and Localization

• MRPL30 is a mitochondrial ribosomal protein that constitutes part of the large 39S subunit, essential for mitochondrial protein synthesis.
• Also known as uL30m, this protein belongs to the universal ribosomal protein uL30 family, conserved across eukaryotes and prokaryotes.
• Encoded by the MRPL30 gene (alternative symbols: MRPL28, RPML28), it undergoes alternative splicing to generate multiple isoforms.
• The protein localizes exclusively to the mitochondrion, directed by an N-terminal transit peptide.
• A 3D-structure is available for this protein, highlighting its role within the ribosomal complex.
• Identified in high-throughput proteomics studies as a component of the mitochondrial ribosome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–161) of human MRPL30, yielding an expected band of ~19 kDa in Western blot under reducing conditions.
• For immunohistochemistry (IHC-P), mitochondrial staining patterns should be observed; consider including appropriate controls such as mitochondrial marker co-staining or antigen blocking.
• This polyclonal antibody cross-reacts with rat MRPL30, making it suitable for comparative studies; however, validation in the specific sample system is recommended.
• As a rabbit polyclonal antibody, batch-to-batch variability may occur; it is advisable to titrate the antibody for each new lot and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, technical services, and consulting from concept to clinic. This anti-MRPL30 polyclonal antibody is part of our extensive mitochondrial and ribosomal protein portfolio, ideal for research into mitochondrial dysfunction and related disorders. Beyond antibodies, we provide validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing to accelerate your diagnostic development.