Anti-MRPL3 Polyclonal Antibody for WB, ELISA - P09001

Core Product Specifications and Parameters

Biological Background: MRPL3 Function and Localization

• MRPL3 encodes the large ribosomal subunit protein uL3m, also known as 39S ribosomal protein L3, mitochondrial (L3mt, MRP-L3).
• It is a component of the mitochondrial large ribosomal subunit, essential for mitochondrial protein synthesis.
• The protein localizes to the mitochondrion, where it participates in the translation of mitochondrial-encoded genes.
• MRPL3 is implicated in primary mitochondrial disease; variants in this gene are associated with combined oxidative phosphorylation deficiency (COXPD9).
• The protein contains a transit peptide for mitochondrial import and has a molecular weight of approximately 38.6 kDa (38633 Da).
• Its 3D structure is available, providing insights into its functional role within the mitochondrial ribosome.
• MRPL3 is a reference proteome component and has been identified through proteomics.

Experimental Guidance and Technical Tips

• The immunogen peptide corresponds to a conserved region within human MRPL3, ensuring specific detection of the target.
• For Western blotting, consider using mitochondrial-enriched protein extracts or whole cell lysates; a band around 38 kDa is expected.
• Validate antibody specificity with appropriate controls, such as MRPL3 knockdown lysates or peptide blocking.
• For ELISA, consider using recombinant MRPL3 protein or peptide as coating antigen; optimize coating buffer and concentration.
• Always include a negative control (e.g., irrelevant rabbit IgG) to assess non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive IVD raw-material solutions, from monoclonal and polyclonal antibodies to ancillary reagents. This anti-MRPL3 antibody supports mitochondrial ribosome and oxidative phosphorylation dysfunction studies, bridging the gap between discovery and clinical assay development. Leverage our validated antibody pairs and rare-target sourcing capabilities to accelerate your diagnostic project.