Anti-MRPL28 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q13084

Core Product Specifications and Parameters

Biological Background: MRPL28 Function and Localization

• MRPL28 encodes the mitochondrial large ribosomal subunit protein bL28m, a core component of the mitochondrial ribosome (mitoribosome) involved in mitochondrial protein synthesis.
• Additionally, MRPL28 is recognized as melanoma antigen p15 (MAAT1), a tumor-associated antigen identified in melanoma, highlighting its potential role in cancer immunity.
• The protein is localized to the mitochondrion, consistent with its function in mitochondrial translation.
• Tissue expression analysis reveals MRPL28 presence in a variety of normal tissues, including spleen, testes, thymus, liver, kidney, brain, adrenal, lung, and retinal tissue.
• UniProt keywords indicate that MRPL28 has a transit peptide for mitochondrial import, has been identified in proteomics studies, and is part of the reference proteome; its 3D structure has been determined (keyword: 3D-structure).
• The calculated molecular weight of the mature protein is approximately 30.2 kDa (UniProt: 30157 Da), aligning with the observed band at 30 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human MRPL28 protein (amino acids 1–256), providing multiple epitope targets typical of polyclonal antibodies; consider this for robust detection.
• For Western blotting under reducing conditions, expect a band near 30 kDa, consistent with the predicted molecular weight.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), incorporate mitochondrial counterstaining or co-localization markers to confirm specific mitochondrial localization.
• For immunoprecipitation, optimize antibody concentration and incubation conditions for your specific sample type.
• This antibody shows cross-reactivity with rat MRPL28, as indicated, making it suitable for studies in rat models; validate in the relevant tissue or cell line.
• As with all polyclonal antibodies, lot-to-lot variability may occur; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, supporting mitochondrial research and cancer biomarker development with validated antibodies like this anti-MRPL28 polyclonal antibody. We offer bulk quantities of optimized monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare-target raw materials, streamlining your transition from research to diagnostic application.