Anti-MRPL28 Monoclonal Antibody for WB, IP, ELISA - Q13084

Core Product Specifications and Parameters

Biological Background: MRPL28 Function and Localization

• MRPL28 encodes the mitochondrial large ribosomal subunit protein bL28m, also known as 39S ribosomal protein L28 (MRP-L28) and melanoma antigen p15 (MAAT1).
• As a component of the mitochondrial ribosome, it participates in mitochondrial protein synthesis, essential for oxidative phosphorylation.
• The protein is identified as a melanoma-associated antigen recognized by T-lymphocytes, suggesting a role in tumor immunology.
• Subcellular localization is exclusively mitochondrial, consistent with its function in mitochondrial translation.
• Tissue expression is broad, detected in spleen, testes, thymus, liver, kidney, brain, adrenal, lung, and retina.
• The protein contains a transit peptide for mitochondrial import and is classified under ribonucleoprotein and ribosomal protein families.
• Structural studies have contributed to the 3D-structure of the mitochondrial ribosome.
• Proteomic identification confirms its presence in the mitochondrial proteome.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100), which includes the transit peptide; the antibody may detect both precursor and mature forms.
• For Western blot, the expected molecular weight is approximately 30 kDa; validate in the relevant sample system as processing may shift the band.
• Cross-reactivity with mouse and rat indicates a conserved epitope; consider using appropriate positive controls from these species.
• For immunoprecipitation, use mitochondrial-enriched lysates to enhance target capture.
• ELISA applications may require optimized coating conditions due to the peptide immunogen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, connecting diagnostic manufacturers, labs, and research institutes with high-quality reagents and technical support from concept to clinic. This anti-MRPL28 monoclonal antibody addresses mitochondrial ribosomal protein and tumor antigen research, and we offer a full portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let us streamline your supply chain and accelerate your diagnostic development.