Anti-MRPL27 Polyclonal Antibody for WB, IHC-P, ELISA - Q9P0M9

Core Product Specifications and Parameters

Biological Background: MRPL27 Function and Localization

• MRPL27 is a constituent of the large (39S) subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis.
• It is encoded by the nuclear gene MRPL27 (GeneID: 51264) and imported into mitochondria, where it assembles into the mitoribosome.
• The protein belongs to the bL27m ribosomal protein family, sharing structural homology with bacterial ribosomal protein L27.
• MRPL27 localizes specifically to the mitochondrion, as indicated by its mitochondrial transit peptide and subcellular fractionation data.
• Post-translational removal of the transit peptide yields the mature 16 kDa protein.
• Structural studies have resolved the 3D architecture of the human mitoribosome, including MRPL27 within the large subunit.

Experimental Guidance and Technical Tips

• The immunogen used is an internal peptide (aa 49-148) of human MRPL27; cross-reactivity with human and mouse is expected. For other species, check sequence alignment.
• For Western blotting, the expected molecular weight is 16 kDa. Use a 12-15% SDS-PAGE gel and optimize transfer conditions for low molecular weight proteins.
• Immunohistochemistry (paraffin) may require antigen retrieval; mitochondrial targets often benefit from heat-induced epitope retrieval and permeabilization.
• ELISA applications can be developed, but titration and specificity checks are recommended.
• Knockdown validation (as indicated) can serve as a specificity control for Western blot; consider including positive and negative lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with streamlined access to premium IVD raw materials. This anti-MRPL27 polyclonal antibody supports mitochondrial biology and protein synthesis research. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your IVD development from concept to clinic.