Anti-MRPL24 Polyclonal Antibody for WB, ELISA - Q96A35

Core Product Specifications and Parameters

Biological Background: MRPL24 Function and Localization

• MRPL24 encodes the mitochondrial large ribosomal subunit protein uL24m (also known as 39S ribosomal protein L24 or MRP-L24), a core component of the mitochondrial ribosome.
• It is essential for mitochondrial protein synthesis, contributing to the translation of the 13 mitochondrially encoded oxidative phosphorylation subunits.
• The protein localizes to the mitochondrion; it contains an N-terminal transit peptide directing its import into the organelle.
• MRPL24 is subject to post-translational modifications including phosphorylation, as indicated by its membership in the Phosphoprotein keyword category.
• The molecular weight of the mature protein is approximately 25 kDa (predicted 24,915 Da), consistent with the observed band in western blotting.
• Its 3D structure has been resolved, providing insights into the architecture of the mitochondrial ribosome.
• MRPL24 has been identified in large-scale proteomics studies, underscoring its role in the mitochondrial ribosomal proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human MRPL24 (amino acids 1–216); use this antibody for western blot analysis under reducing conditions, expecting a specific band near 25 kDa.
• For ELISA applications, titrate the antibody to optimize signal-to-noise ratio, and validate with recombinant or endogenous MRPL24 protein.
• Due to cross-reactivity with mouse and rat, this antibody can be used in studies across these species; however, confirm specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing platform for IVD raw materials, CamelBio facilitates your mitochondrial research by providing validated antibodies like this anti-MRPL24 polyclonal antibody, alongside matched recombinant proteins and technical consultation. Our expertise extends to bulk supply of ancillary reagents and custom sourcing for rare targets, supporting diagnostic manufacturers from development to commercialization.