Anti-MRPL22 Polyclonal Antibody for WB, ELISA - Q9NWU5

Core Product Specifications and Parameters

Biological Background: MRPL22 Function and Localization

• MRPL22 (mitochondrial ribosomal protein L22), also known as L22mt, MRP-L22, or MRP-L25, is a nucleus-encoded protein that localizes to the mitochondria after synthesis.
• It is a component of the large (39S) subunit of the mitochondrial ribosome, where it plays an essential role in the translation of mitochondrial DNA-encoded proteins.
• The mitochondrial ribosome is responsible for synthesizing 13 protein subunits of the oxidative phosphorylation complexes, critical for ATP production.
• Alternative splicing of the MRPL22 gene produces multiple protein isoforms; the UniProt entry reports a canonical molecular weight of approximately 23.6 kDa, while additional isoforms of 13 kDa and 14 kDa have been observed.
• Structural studies (3D-structure keyword) have provided insights into the organization of this ribosomal protein within the mitoribosome.
• A N-terminal transit peptide directs the nascent protein across the mitochondrial membranes; this peptide is cleaved upon import, generating the mature ribosomal protein.
• As a member of the ribosomal protein family, MRPL22 is classified under ribonucleoproteins and participates in mitochondrial protein synthesis.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 59–206) covers a central region of human MRPL22 that is conserved across multiple isoforms, which may lead to detection of multiple bands in Western blot.
• For Western blot, consider using mitochondrial-enriched extracts or whole-cell lysates from human cell lines. The expected molecular weights (13, 14, and 23 kDa) should be monitored.
• As a polyclonal antibody, batch-to-batch consistency and optimal dilution should be determined by the end user. Validate reactivity in the relevant sample system before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material supplier, offering validated antibodies and bulk ancillary reagents to support mitochondrial research and protein synthesis studies. For projects targeting MRPL22, we provide ready-to-use polyclonal antibodies and can assist with custom monoclonal antibody development, antigen-grade proteins, and rare-target sourcing. Trust CamelBio to bridge your concept-to-clinic pipeline with reliable, scalable raw-material solutions.