Anti-MRPL21 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q7Z2W9

Core Product Specifications and Parameters

Biological Background: MRPL21 Function and Localization

• MRPL21 encodes the large ribosomal subunit protein bL21m, a component of the mitochondrial ribosome (mitoribosome) 39S subunit.
• The protein is also known as 39S ribosomal protein L21, mitochondrial (L21mt or MRP-L21).
• Mitoribosomes are responsible for translating the 13 mitochondrial DNA-encoded proteins essential for oxidative phosphorylation.
• According to UniProt, the protein localizes exclusively to the mitochondrion, consistent with its role in mitochondrial translation.
• It contains a transit peptide that directs it to the mitochondria, where it is processed to the mature form.
• Alternative splicing of the MRPL21 gene generates multiple transcript variants (UniProt keyword: Alternative splicing).
• The protein has been identified in high-throughput proteomics studies (Proteomics identification) and its 3D structure has been partially resolved (3D-structure keyword).
• Predicted molecular weight is approximately 22.8 kDa (mature form), consistent with the reported 22 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 40-140) of human MRPL21, likely corresponding to a surface-exposed loop in the mature ribosomal protein, making it suitable for detecting both native and denatured protein.
• For WB, a band at approximately 22 kDa is expected in mitochondrial-enriched lysates; consider using such lysates for optimal detection.
• For IHC-P, mitochondrial-rich tissues such as heart or skeletal muscle may provide strong staining; validate in the relevant sample system.
• ELISA can be used for peptide or protein quantification; ensure appropriate coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials for mitochondrial translation research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for MRPL21 and related mitochondrial ribosomal proteins. Whether you need a ready-to-use detection antibody or custom development support, our team ensures scientists and manufacturers can bridge the gap from concept to clinic efficiently.